The serodiagnosis of infections caused by Verocytotoxin-producing Escherichia coli
- PMID: 10347867
- DOI: 10.1046/j.1365-2672.1999.00766.x
The serodiagnosis of infections caused by Verocytotoxin-producing Escherichia coli
Abstract
Patients with haemolytic uraemic syndrome (HUS) and haemorrhagic colitis (HC) produce serum antibodies to the lipopolysaccharides (LPS) of Escherichia coli O157 and certain other E. coli serogroups. Patients may also make salivary antibodies to the LPS of E. coli O157. Serological tests based on these antibodies can be used to provide evidence of infection in the absence of culturable VTEC or the toxins they produce. Serum antibodies to LPS persist for several months following onset of disease, enabling both current and retrospective serological testing. The LPS of E. coli O157 shares epitopes with strains of Brucella abortus, Yersinia enterocolitica O9, Vibrio cholerae O1 Inaba, group N Salmonella and certain strains of Citrobacter freundii and E. hermanni. Serological tests for serum antibodies to E. coli O157 should be evaluated in the light of these cross-reactions. Serological tests to supply evidence of infection with E. coli O157 have been shown to provide a valuable adjunct to bacteriological procedures for detecting culturable VTEC and VT. The use of well characterized LPS antigens in association with the techniques of ELISA and immunoblotting provide valuable procedures for detecting evidence of infection with E. coli O157 and possibly other VTEC.
Similar articles
-
Human antibody responses to R3-core epitopes on the lipopolysaccharide of Escherichia coli O157.Lett Appl Microbiol. 2003;37(6):429-32. doi: 10.1046/j.1472-765x.2003.01391.x. Lett Appl Microbiol. 2003. PMID: 14633093
-
The serological response to Verocytotoxigenic Escherichia coli in patients with haemolytic uraemic syndrome.Lett Appl Microbiol. 2004;38(5):351-4. doi: 10.1111/j.1472-765X.2004.01499.x. Lett Appl Microbiol. 2004. PMID: 15059202
-
Verocytotoxin-producing Escherichia coli infection in household members of children with hemolytic-uremic syndrome in The Netherlands.Pediatr Infect Dis J. 1999 Aug;18(8):709-14. doi: 10.1097/00006454-199908000-00011. Pediatr Infect Dis J. 1999. PMID: 10462341
-
[Hemolytic uremic syndrome associated with entero-hemorrhagic Escherichia coli].Nihon Rinsho. 1993 Jan;51(1):198-203. Nihon Rinsho. 1993. PMID: 8433519 Review. Japanese.
-
[Sporadic cases of hemolytic uremic syndrome and hemorrhagic colitis with serum IgM antibodies to lipopolysaccharides of enterohemorrhagic Escherichia coli O157].Nihon Rinsho. 1997 Mar;55(3):686-92. Nihon Rinsho. 1997. PMID: 9086782 Review. Japanese.
Cited by
-
The emerging importance of Shiga toxin-producing Escherichia coli other than serogroup O157 in England.J Med Microbiol. 2021 Jul;70(7):001375. doi: 10.1099/jmm.0.001375. J Med Microbiol. 2021. PMID: 34309502 Free PMC article.
-
Shiga toxins and the pathophysiology of hemolytic uremic syndrome in humans and animals.Toxins (Basel). 2012 Nov 8;4(11):1261-87. doi: 10.3390/toxins4111261. Toxins (Basel). 2012. PMID: 23202315 Free PMC article. Review.
-
Risk factors for poor renal prognosis in children with hemolytic uremic syndrome.Pediatr Nephrol. 2003 Dec;18(12):1229-35. doi: 10.1007/s00467-003-1262-6. Epub 2003 Oct 31. Pediatr Nephrol. 2003. PMID: 14593522
-
Fecal diagnostics in combination with serology: best test to establish STEC-HUS.Pediatr Nephrol. 2016 Nov;31(11):2163-70. doi: 10.1007/s00467-016-3420-7. Epub 2016 May 30. Pediatr Nephrol. 2016. PMID: 27240858 Free PMC article.
-
Childhood hemolytic uremic syndrome, United Kingdom and Ireland.Emerg Infect Dis. 2005 Apr;11(4):590-6. doi: 10.3201/eid1104.040833. Emerg Infect Dis. 2005. PMID: 15829199 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical