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. 1999 Apr 16;726(1-2):313-6.
doi: 10.1016/s0378-4347(99)00060-2.

High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat

T Sakai  1 , A Habiro, S Kawahara
Affiliations

High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat

T Sakai et al. J Chromatogr B Biomed Sci Appl. .

Abstract

The reaction conditions of 1,3-diethyl-2-thiobarbituric acid (DETBA)-malonaldehyde (MA) adduct formation were examined in order to analyze MA in fish tissue by high-performance liquid chromatography. A reaction mixture containing 4 mM butyl hydroxytoluene was heated at 100 degrees C for 150 min and the DETBA-MA adduct formed was separated by a Inertsil ODS column for 20 min. The detection limit was 5 pmol.

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