doi: 10.1016/s0378-4347(99)00060-2.
High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat
Affiliations
- PMID: 10348202
- DOI: 10.1016/s0378-4347(99)00060-2
Item in Clipboard
High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat
J Chromatogr B Biomed Sci Appl.
.
Abstract
The reaction conditions of 1,3-diethyl-2-thiobarbituric acid (DETBA)-malonaldehyde (MA) adduct formation were examined in order to analyze MA in fish tissue by high-performance liquid chromatography. A reaction mixture containing 4 mM butyl hydroxytoluene was heated at 100 degrees C for 150 min and the DETBA-MA adduct formed was separated by a Inertsil ODS column for 20 min. The detection limit was 5 pmol.
Similar articles
-
Peroxyoxalate chemiluminescence detection of condensates of malondialdehyde with thiobarbituric acids using a flow system.Biomed Chromatogr. 1992 Mar-Apr;6(2):55-8. doi: 10.1002/bmc.1130060202. Biomed Chromatogr. 1992. PMID: 1638090
-
Malondialdehyde measurement in oxidized foods: evaluation of the spectrophotometric thiobarbituric acid reactive substances (TBARS) test in various foods.J Agric Food Chem. 2012 Sep 26;60(38):9589-94. doi: 10.1021/jf302451c. Epub 2012 Sep 17. J Agric Food Chem. 2012. PMID: 22950760
-
Determination of malonaldehyde in oxidized biological materials by high-performance liquid chromatography.J Chromatogr. 1990 Aug 31;515:391-7. doi: 10.1016/s0021-9673(01)89334-1. J Chromatogr. 1990. PMID: 2283368
-
Quantification of malondialdehyde by HPLC-FL - application to various biological samples.Biomed Chromatogr. 2015 Jan;29(1):41-6. doi: 10.1002/bmc.3361. Epub 2014 Oct 30. Biomed Chromatogr. 2015. PMID: 25355691 Review.
-
The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress.Life Sci. 1991;48(4):301-9. doi: 10.1016/0024-3205(91)90550-u. Life Sci. 1991. PMID: 1990230 Review.