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. 1999 Jun;43(6):1500-2.
doi: 10.1128/AAC.43.6.1500.

Interstrain variation in the human cytomegalovirus DNA polymerase sequence and its effect on genotypic diagnosis of antiviral drug resistance. Adult AIDS Clinical Trials Group CMV Laboratories

S Chou  1 , N S Lurain, A Weinberg, G Y Cai, P L Sharma, C S Crumpacker
Affiliations

Interstrain variation in the human cytomegalovirus DNA polymerase sequence and its effect on genotypic diagnosis of antiviral drug resistance. Adult AIDS Clinical Trials Group CMV Laboratories

S Chou et al. Antimicrob Agents Chemother. 1999 Jun.

Abstract

The polymerase (pol) coding sequence was determined for 40 independent clinical cytomegalovirus isolates sensitive to ganciclovir and foscarnet. Sequence alignments showed >98% interstrain homology and amino acid variation in only 4% of the 1, 237 codons. Almost all variation occurred outside of conserved functional domains where resistance mutations have been identified.

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Figures

FIG. 1
FIG. 1
CMV DNA polymerase mutation map. Shown at top are the conserved functional domains and their codon ranges (boxed). Loci of amino acid changes are mapped below. (a) Codons showing variation in drug-sensitive isolates in this study; (b) codons mapped to drug resistance in laboratory strains (8, 12, 14); (c) codons mapped to drug resistance in clinical isolates (, –7, 9, 11, 13); (d) drug resistance phenotypes associated with drug-resistant mutants according to region. Regions known to be associated with drug resistance are shaded. GCV, ganciclovir; CDV, cidofovir; PFA, foscarnet.
See this image and copyright information in PMC

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