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Comparative Study
. 1999 Jun;181(11):3594-8.
doi: 10.1128/JB.181.11.3594-3598.1999.

Comparison of the heme iron utilization systems of pathogenic Vibrios

S M O'Malley  1 , S L Mouton, D A Occhino, M T Deanda, J R Rashidi, K L Fuson, C E Rashidi, M Y Mora, S M Payne, D P Henderson
Affiliations
Comparative Study

Comparison of the heme iron utilization systems of pathogenic Vibrios

S M O'Malley et al. J Bacteriol. 1999 Jun.

Abstract

Vibrio alginolyticus, Vibrio fluvialis, and Vibrio parahaemolyticus utilized heme and hemoglobin as iron sources and contained chromosomal DNA similar to several Vibrio cholerae heme iron utilization genes. A V. parahaemolyticus gene that performed the function of V. cholerae hutA was isolated. A portion of the tonB1 locus of V. parahaemolyticus was sequenced and found to encode proteins similar in amino acid sequence to V. cholerae HutW, TonB1, and ExbB1. A recombinant plasmid containing the V. cholerae tonB1 and exbB1D1 genes complemented a V. alginolyticus heme utilization mutant. These data suggest that the heme iron utilization systems of the pathogenic vibrios tested, particularly V. parahaemolyticus and V. alginolyticus, are similar at the DNA level, the functional level, and, in the case of V. parahaemolyticus, the amino acid sequence or protein level to that of V. cholerae.

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Figures

FIG. 1
FIG. 1
Autoradiograms of Southern blots of Vibrio strains probed with V. cholerae tonB1 (A) and tonB2 (B). The following strains were tested: V. cholerae CA401 (lane 1); V. parahaemolyticus 474801, M47314, and 115 (lanes 2 to 4, respectively); V. alginolyticus BE2-542 (lane 5); and V. fluvialis BE2-819 (lane 6). Size markers are indicated on the left.
FIG. 2
FIG. 2
Genetic map of cloned DNA from pPHU2. The putative Fur box is indicated by an open box. The arrows labeled P1 and P2 indicate the locations of the proposed promoters for phuW and for tonB1 and exbB1, respectively. The arrows beneath the filled boxes denote the direction of transcription. Below the map is the DNA sequence containing the predicted divergent promoters and the proposed Fur box, which is marked with a thick black line above the DNA.
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