Degradation of oat mRNAs during seed development

Abstract

The genes AV1, AV10, and Z1 encode proteins that accumulate during oat seed development. In developing endosperm of Avena sativa (cultivated oat), AV1, AV10 and Z1 mRNAs reach maximal levels midway through seed development but fall to very low levels in mature seeds. Similarly, mRNAs for these proteins peak during endosperm development of Avena fatua (wild oat) and are later degraded. However, during late maturation of A. fatua seeds, populations of mRNA fragments shorter than the intact transcripts accumulate as the full-length transcripts decline in abundance. The smaller RNA molecules, which are apparently long-lived decay intermediates, are derived randomly from the entire transcripts and are most likely not generated by cleavage at precisely defined sites. Other A. fatua endosperm mRNAs that are degraded during late seed development, such as those for ADP glucose pyrophosphorylase and starch synthase, do not produce detectable decay intermediates. Decay intermediates of AV1 and Z1 mRNAs persist at high levels during late seed development of two other undomesticated oat species, Avena strigosa and Avena barbata. The persistence of decay intermediates for these endosperm mRNAs in wild grass species may represent a model system for studying RNA decay process in plant tissues.