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. 1999 Apr;122(2):337-41.
doi: 10.1017/s0950268899002198.

Molecular typing of Escherichia coli O157:H7 (H-) isolates from cattle in Japan

M Akiba  1 , T Masuda, T Sameshima, K Katsuda, M Nakazawa
Affiliations

Molecular typing of Escherichia coli O157:H7 (H-) isolates from cattle in Japan

M Akiba et al. Epidemiol Infect. 1999 Apr.

Abstract

A total of 77 Escherichia coli O157:H7 (H-) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx-2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157:H7 infection in Japan.

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