Cellular roles in relapse processes of experimentally-moved rat molars

Abstract

This histological study was designed to clarify the relapse processes of rat molars following orthodontic tooth movement by the Waldo method. An elastic band was inserted between the upper first and second molars, and removed either 7 or 21 days later. The relapse processes of first molars were examined at 1 or 4 days after completing the 7-day-insertion and at 5 or 10 days after the end of the 21-day-insertion. At the end of the 7-day-insertion period, the mean interdental space was 158 microns, but decreased rapidly, reaching 44 microns by 1 day after removal, and 16 microns by day 4. In the periodontal ligament (PDL) compressed by elastic band insertion, partial hyalinization and/or mineralization occurred. The stretched PDL consisted of irregularly-distributed, thin principal fibres, approximately 2.4 microns in diameter. On days 1 and 4 after removal, osteoclasts, macrophage-like cells and fibroblast-like cells were localized around hyalinized tissues, but thick principal fibres, approximately 3.8 microns in diameter, were regularly distributed throughout the PDL proper. The mean interdental space after the 21-day-insertion was 536 microns, but this had decreased to 108 microns by day 5, and 71 microns by day 10. On day 5 after removal, numerous osteoclasts were evenly distributed in the compressed PDL, while thick principal fibres, approximately 3.5 microns, were present in the stretched PDL. These results suggest that, during relapse of experimentally-moved rat molars, (i) rapid remodelling of the PDL and surrounding alveolar bones is the main cause of tooth relapse and (ii) hyalinized and/or mineralized tissues occurred by compression are rapidly resorbed by osteoclasts, macrophage-like cells and fibroblast-like cells.