Isolation of a high specific activity 35S ribosomal RNA precursor from Tetrahymena pyriformis and identification of its 5' terminus, pppAp
- PMID: 103568
- DOI: 10.1021/bi00615a035
Isolation of a high specific activity 35S ribosomal RNA precursor from Tetrahymena pyriformis and identification of its 5' terminus, pppAp
Abstract
RNA isolated from starved and refed Tetrahymena pyriformis GL, pulse labeled with H3(32)PO4, contains an RNA molecule of 2.4 x 10(6) molecular weight which can be identified by polyacrylamide gel electrophoresis. This RNA has been shown to be a precursor of the mature 25S rRNA and 17S rRNA by a kinetic analysis of its synthesis, by hybridization competition with pure 25S rRNA and 17s rRNA, and by two-dimensional fingerprint analysis. The 5'-terminal nucleotide was isolated from the total digestion products of the 35S rRNA precusor and shown to be pppAp by analysis of the products of snake venom phosphodiesterase digestion. Low, but significant yields of the 5' terminus pppAp were also isolated from the 17S rRNA demonstrating that the primary transcript of the rRNA gene in T. pyriformis contains the 17S rRNA at its 5' end.
Similar articles
-
Physical map of the ribosomal ribonucleic acid gene from Tetrahymena pyriformis.Biochemistry. 1981 Feb 17;20(4):905-9. doi: 10.1021/bi00507a039. Biochemistry. 1981. PMID: 6260143
-
5' -and 3'-terminal nucleotide sequences of Tetrahymena pyriformis 17S rRNA.Biochemistry. 1977 May 31;16(11):2380-3. doi: 10.1021/bi00630a011. Biochemistry. 1977. PMID: 405038
-
RNA synthesis in division synchronized Tetrahymena: macronuclear and cytoplasmic RNA.J Protozool. 1976 Nov;23(4):594-600. doi: 10.1111/j.1550-7408.1976.tb03849.x. J Protozool. 1976. PMID: 826626
-
Poly(A)+ RNA from Tetrahymena: stimulation of protein synthesis in vitro.J Protozool. 1979 Nov;26(4):672-5. doi: 10.1111/j.1550-7408.1979.tb04218.x. J Protozool. 1979. PMID: 94610
-
Nucleic acids of Entamoeba histolytica.J Protozool. 1989 Mar-Apr;36(2):197-205. doi: 10.1111/j.1550-7408.1989.tb01074.x. J Protozool. 1989. PMID: 2470900 Review.
Cited by
-
The nucleotide sequence at the transcription termination site of the ribosomal RNA gene in Tetrahymena thermophila.Nucleic Acids Res. 1982 Mar 11;10(5):1503-13. doi: 10.1093/nar/10.5.1503. Nucleic Acids Res. 1982. PMID: 6280147 Free PMC article.
-
Determination of the transcription initiation site of Tetrahymena pyriformis rDNA using in vitro capping of 35S pre-rRNA.Nucleic Acids Res. 1982 Jul 24;10(14):4223-36. doi: 10.1093/nar/10.14.4223. Nucleic Acids Res. 1982. PMID: 6289268 Free PMC article.
-
Complex endonucleolytic cleavage pattern during early events in the processing of pre-rRNA in the lower eukaryote, Tetrahymena thermophila.Nucleic Acids Res. 1983 Jun 11;11(11):3487-502. doi: 10.1093/nar/11.11.3487. Nucleic Acids Res. 1983. PMID: 6304633 Free PMC article.
-
Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.Proc Natl Acad Sci U S A. 1979 Oct;76(10):5051-5. doi: 10.1073/pnas.76.10.5051. Proc Natl Acad Sci U S A. 1979. PMID: 291921 Free PMC article.
-
Processing of ribosomal precursor RNAs in Physarum polycephalum.Nucleic Acids Res. 1980 Jun 25;8(12):2647-64. doi: 10.1093/nar/8.12.2647. Nucleic Acids Res. 1980. PMID: 6253887 Free PMC article.