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. 1999 Jun;16(3):141-4.

[Screening for mitochondrial 1555(G) mutation in patients with aminoglycoside antibiotic-induced deafness]

[Article in Chinese]
H Yuan  1 , S Jiang, W Yang, W Guo, J Cao, P Dai
Affiliations
  • PMID: 10359861

[Screening for mitochondrial 1555(G) mutation in patients with aminoglycoside antibiotic-induced deafness]

[Article in Chinese]
H Yuan et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Jun.

Abstract

Objective: To identify the incidence of the 1555(G) mutation in pedigrees and sporadic patients with aminoglycoside antibiotic- induced deafness so as, to privide the theoretical evidence for establishing the method of diagnosis of this disease.

Methods: Blood samples were obtained from two pedigrees and seven sporadic patients with aminoglycoside antibiotic-induced deafness, and five mothers of the sporadic patients. DNA was extracted from the isolated leukocytes. The mitochondrial DNA fragments were amplified by PCR; 1555(G) mutation was detected by Alw26 I restriction endonuclease digestion.

Results: Fourteen individuals from two pedigrees carried homoplasmic 1555(G) mutation. Seven sporadic patients and the five mothers did not have 1555(G) mutation.

Conclusion: The incidence of the 1555(G) mutation in pedigrees with aminoglycoside antibiotic-induced deafness is fairly high, while in sporadic patients is low. Screening for mitochondrial 1555(G) mutation is of potential value to clinical use.

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