Histological damage in chronic hepatitis C is not related to the extent of infection in the liver
- PMID: 10362814
- PMCID: PMC1866612
- DOI: 10.1016/S0002-9440(10)65445-4
Histological damage in chronic hepatitis C is not related to the extent of infection in the liver
Abstract
It has not been completely elucidated whether the liver injury induced by the hepatitis C virus (HCV) is due to direct cytopathic damage or to an immune-mediated response against HCV-infected hepatocytes. In this work, we have determined the percentage of HCV-infected hepatocytes, the histological activity index, and the viremia levels in chronically HCV-infected patients with different grades of liver injury to investigate any possible correlation between them. For that purpose, liver biopsies from 27 patients with HCV chronic hepatitis were analyzed by in situ hybridization. This technique revealed that the percentage of infected hepatocytes ranged from 0.04% to 83.6%. Regarding the viremia levels, HCV RNA concentration ranged from 1.8 x 10(3) to 1.4 x 10(6) genome copies/ml. A significant correlation (r = 0.54; P = 0.003) between the percentage of infected hepatocytes and the viremia levels was found. In contrast, no correlation was observed between the percentage of HCV-infected hepatocytes or the viremia levels and the histological activity index. In conclusion, we have shown that the HCV viremia reflects the extent of the infection in the liver and that the liver injury in chronic HCV infection is not directly related to either the number of infected hepatocytes or the serum HCV RNA concentration.
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References
-
- Shindo M, Di Bisceglie AM, Biswas R, Mihalik K, Feinstone SM: Hepatitis C virus replication during acute infection in the chimpanzee. J Infect Dis 1992, 166:424-427 - PubMed
-
- Kato N, Yokosuka O, Hosoda K, Ito Y, Onto M, Omata M: Quantification of hepatitis C virus by competitive reverse transcription-polymerase chain reaction: increase of the virus in advanced liver disease. Hepatology 1993, 18:16-20 - PubMed
-
- Yuki N, Hayashi N, Kamada T: HCV viraemia and liver injury in symptom-free blood donors [letter]. Lancet 1993, 342:444 - PubMed
-
- Di Bisceglie AM, Hoofnagle JH, Krawczynski K: Changes in hepatitis C virus antigen in liver with antiviral therapy. Gastroenterology 1993, 105:858-862 - PubMed
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