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. 1976 Dec;33(3):517-21.
doi: 10.1099/0022-1317-33-3-517.

Purification of interferon by adsorption chromatography on controlled pore glass

V G Edy, I A Braude, E De Clercq, A Billiau, P De Somer

Purification of interferon by adsorption chromatography on controlled pore glass

V G Edy et al. J Gen Virol. 1976 Dec.

Abstract

Human fibroblast and mouse L929 cell interferons can be purified by adsoprtion to and subsequent elution from Controlled Pore Glass. Purification of 40 to 90-fold to specific activities of 1 to 5 times 10(6) units/mg of protein can be achieved in a single step, with good recovery of activity. Human leukocyte interferon does not bind to the glass and cannot be purified in this way.

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