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Multicenter Study
. 1999 Jul;37(7):2165-9.
doi: 10.1128/JCM.37.7.2165-2169.1999.

Most Enterobacter aerogenes strains in France belong to a prevalent clone

C Bosi  1 , A Davin-Regli, C Bornet, M Mallea, J M Pages, C Bollet
Affiliations
Multicenter Study

Most Enterobacter aerogenes strains in France belong to a prevalent clone

C Bosi et al. J Clin Microbiol. 1999 Jul.

Abstract

The aim of this study was to determine the distribution in France of the Enterobacter aerogenes prevalent clone isolated in the hospitals of the Marseille area (A. Davin-Regli, D. Monnet, P. Saux, C. Bosi, R. Charrel, A. Barthelemy, and C. Bollet, J. Clin. Microbiol. 34:1474-1480, 1996). A total of 123 E. aerogenes isolates were collected from 23 hospital laboratories and analyzed by random amplification of polymorphic DNA and enterobacterial repetitive intergenic consensus-PCR to determine their epidemiological relatedness. Molecular typing revealed that 21 of the 23 laboratories had isolated this prevalent clone harboring the plasmid encoding for extended-spectrum beta-lactamase of the TEM-24 type. Most isolates were susceptible only to imipenem and gentamicin. Their dissemination seems to be clonal and was probably the result of the general use of broad-spectrum cephalosporins and quinolones. Four isolates showed an alteration of their outer membrane proteins, causing decrease of susceptibility to third-generation cephalosporins and imipenem and leading to the critical situation of having no alternative therapeutic. The large dissemination of the E. aerogenes prevalent clone probably results from its good adaptation to the antibiotics administered in France and the hospital environment, particularly in intensive care units.

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Figures

FIG. 1
FIG. 1
ERIC-PCR (upper panel) and RAPD (lower panel) fingerprints of 40 Enterobacter aerogenes isolates from hospital laboratories. Lane M, molecular weight marker (marker VI).
FIG. 2
FIG. 2
Representation of the dissemination of the E. aerogenes prevalent clone among 23 hospital centers in France (prevalent clone/total collected strains); ⧫ represents porin-deficient phenotype.
FIG. 3
FIG. 3
Immunodetection of E. aerogenes outer membrane proteins. Immunostaining was done with polyclonal antibodies directed against the OmpF monomer (a and b) or OmpC (c and d). Strains PMY150 (P) and BZB1107 (B) were used as positive and negative samples, respectively. Strains from Aubagne (lanes 3 and 5), Le Mans (lane 8), and Nancy (lane 10) were the four strains that presented a porin-deficient phenotype. Lane numbers do not correspond to those in Fig. 1. Only the relevant part of the blot is shown. The 34-kDa arrow indicates the postulated porin migration.
See this image and copyright information in PMC

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