N-ras mutations in 43 Chinese cases of acute myeloid leukemia

Abstract

Objective: To detect 3 kinds of N-ras mutations in Chinese patients with acute myeloid leukemia (AML).

Methods: In vitro DNA amplification followed by oligonucleotide dot analysis were used to study N-ras gene mutations in 43 cases of acute myeloid leukemia (AML). 25 healthy people were used as controls. Patients were selected in the Beijing district and consisted of 19 males and 24 females. The average age was 37. The controls were healthy individuals with the average age of 36.5 from the same region. 3 oligonucleotide probes were artificially synthesized to detect mutations in codon 12 and 13 of N-ras.

Results: Five out of 43 AML samples have been found contain G-->A mutation in codon 12.2 have G-->T mutation in codon 12. One has G-->A mutation in codon 13. The mutation rate was 18.6%. None of the controls presented these mutations. The frequency of mutation of N-ras in the AML samples showed statistical differences with that of the controls.

Conclusion: Analysis of the results suggests the N-ras mutations may have some relationship with the etiology of acute myeloid leukemia.