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. 1999 May;99(1):1-9.
doi: 10.1016/s0009-3084(99)00002-x.

Long-lifetime lipid rhenium metal-ligand complex for probing membrane dynamics on the microsecond timescale

L Li  1 , F N Castellano, I Gryczynski, J R Lakowicz
Affiliations

Long-lifetime lipid rhenium metal-ligand complex for probing membrane dynamics on the microsecond timescale

L Li et al. Chem Phys Lipids. 1999 May.

Abstract

We report the luminescence and spectral properties of a phospholipid analogue containing a long-lifetime luminescent rhenium metal-ligand complex (MLC) covalently linked to the amino group of phosphatidyl ethanolamine. When incorporated into synthetic membranes, this lipid probe displays intensity decay times near 3 microseconds. Importantly, the probe displays highly polarized emission with a maximal fundamental anisotropy of 0.33. This probe is expected to have numerous applications for studies of microsecond diffusion and dynamics of membranes.

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Figures

Fig. 1.
Fig. 1.
Absorption and emission spectra of Re-PE in DPPG vesicles at 20°C. Also shown is the excitation anisotropy spectrum of the parent compound [Re(,4,7-Me2phen)(CO)3(4-COOHPy)]+ in glycerol/methanol at −60°C.
Fig. 2.
Fig. 2.
Frequency-domain intensity decays of Re-PE incorporated in DPPG vesicles at various temperatures.
Fig. 3.
Fig. 3.
Frequency-domain anisotropy decays of DPPG vesicles labeled with Re-PE.
Fig. 4.
Fig. 4.
Reconstructed time-domain anisotropy, decays of DPPG vesicles labeled with Re-PE.
Fig. 5.
Fig. 5.
Frequency-domain intensity decay of Re(Me2-phen)(CO)3 (COOHPy) PF6 in glycerol.
Fig. 6.
Fig. 6.
Frequency-domain anisotropy decay of Re(Me2-phen)(CO)3 (COOHPy) PF6 in glycerol.
Scheme I.
Scheme I.
Molecular structure of the phospholipid analogue of [Re(4,7-Me2phen)(CO)3(4-COOHPy)]+(Re-PE).0
See this image and copyright information in PMC

References

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