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. 1978 Dec 1;58(3):183-92.
doi: 10.1007/BF00495718.

Immunohistochemical demonstration of carbonic anhydrase

T Kumpulainen, L K Korhonen

Immunohistochemical demonstration of carbonic anhydrase

T Kumpulainen et al. Histochemistry. .

Abstract

Rabbits were immunized using human erythroxyte carbonic anhydrase B (HCA B) purified by the modified methods of Armstrong et al. (1966) and Bernstein and Schraer (1972). The globulin fraction was isolated by ammonium sulphate precipitation. The anti-HCA B globulin was specific, when judged using the double diffusion technique of Ouchterlony and immunoelectrophoresis. No cross reaction with human erythrocyte carbonic anhydrase C was found, but cross reactions with erythrocyte carbonic anhydrase from rat, mouse and guinea pig were observed. Flurorescein isothiocyanate conjugated goat anti-rabbit globulin was used for the localization of HCA B in tissue sections and erythrocytes on slides.

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