Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Dec 1;58(3):183-92.
doi: 10.1007/BF00495718.

Immunohistochemical demonstration of carbonic anhydrase

T Kumpulainen, L K Korhonen

Immunohistochemical demonstration of carbonic anhydrase

T Kumpulainen et al. Histochemistry. .

Abstract

Rabbits were immunized using human erythroxyte carbonic anhydrase B (HCA B) purified by the modified methods of Armstrong et al. (1966) and Bernstein and Schraer (1972). The globulin fraction was isolated by ammonium sulphate precipitation. The anti-HCA B globulin was specific, when judged using the double diffusion technique of Ouchterlony and immunoelectrophoresis. No cross reaction with human erythrocyte carbonic anhydrase C was found, but cross reactions with erythrocyte carbonic anhydrase from rat, mouse and guinea pig were observed. Flurorescein isothiocyanate conjugated goat anti-rabbit globulin was used for the localization of HCA B in tissue sections and erythrocytes on slides.

PubMed Disclaimer

References

    1. Arch Biochem Biophys. 1970 Feb;136(2):361-7 - PubMed
    1. Anal Biochem. 1973 Jan;51(1):288-96 - PubMed
    1. Ann N Y Acad Sci. 1968 Jun 14;151(1):64-77 - PubMed
    1. Acta Physiol Scand. 1974 Jan;90(1):143-52 - PubMed
    1. Clin Chim Acta. 1968 Mar;19(3):499-505 - PubMed