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. 1999 Jul;65(7):3240-3.
doi: 10.1128/AEM.65.7.3240-3243.1999.

In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay

J L Williamson  1 , T E Rocke, J M Aiken
Affiliations

In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay

J L Williamson et al. Appl Environ Microbiol. 1999 Jul.

Abstract

A nested PCR was developed for detection of the Clostridium botulinum type C1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.

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Figures

FIG. 1
FIG. 1
Detection of the C1 toxin gene amplification products in DNA samples isolated from sediments collected at botulism outbreak and nonoutbreak sites. (A) ToxC-384/850R/625 primer combination; (B) ToxC-625/1049R/850R primer combination. Images were generated with Photoshop version 5.02 and Freehand version 7.02 software. Letters and numbers above the lanes are sample designations. NTC, no-template control.
FIG. 2
FIG. 2
Detection of the C1 toxin gene in strain 96 SAC bacterial cells or spores added to sediment sample K7A prior to DNA extraction and purification. Images were generated with Photoshop version 5.02 and Freehand version 7.02 software.
See this image and copyright information in PMC

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