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. 1999 Jul;6(4):627-9.
doi: 10.1128/CDLI.6.4.627-629.1999.

Brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family Rhizobiaceae

A Cloeckaert  1 , A Tibor, M S Zygmunt
Affiliations

Brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family Rhizobiaceae

A Cloeckaert et al. Clin Diagn Lab Immunol. 1999 Jul.

Abstract

Brucellae have been reported to be phylogenetically related to bacteria of the family Rhizobiaceae. In the present study, we used a panel of monoclonal antibodies (MAbs) to Brucella outer membrane proteins (OMPs) to determine the presence of common OMP epitopes in some representative bacteria of this family, i.e., Ochrobactrum anthropi, Phyllobacterium rubiacearum, Rhizobium leguminosarum, and Agrobacterium tumefaciens, and also in bacteria reported to serologically cross-react with brucella, i.e., Yersinia enterocolitica O:9, Escherichia coli O:157, and Salmonella urbana. In particular, most MAbs to the Brucella outer membrane lipoproteins Omp10, Omp16, and Omp19 cross-reacted with O. anthropi and P. rubiacearum, which are actually the closest relatives of brucellae. Some of them also cross-reacted, but to a lower extent, with R. leguminosarum and A. tumefaciens. The putative Omp16 and Omp19 homologs in these bacteria showed the same apparent molecular masses as their Brucella counterparts. None of the antilipoprotein MAbs cross-reacted with Y. enterocolitica O:9, E. coli O:157, or S. urbana.

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Figures

FIG. 1
FIG. 1
Reactivity in immunoblotting of anti-Omp10 (lane 1), anti-Omp16 (lanes 2 to 6), and anti-Omp19 MAbs (lanes 7 to 9) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of B. abortus 544 (lanes 1, 2, and 7), O. anthropi (strains 3301 and 3331 gave the same result) (lanes 3 and 8), P. rubiacearum (lanes 4 and 9), A. tumefaciens (lane 5), and R. leguminosarum (lane 6).
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