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. 1999 Jul;6(4):630-2.
doi: 10.1128/CDLI.6.4.630-632.1999.

A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds

G Leiner  1 , B Franz, K Strutzberg, G F Gerlach
Affiliations

A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds

G Leiner et al. Clin Diagn Lab Immunol. 1999 Jul.

Abstract

For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.

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Figures

FIG. 1
FIG. 1
ELISA reactivity patterns of sera from five groups of 10 animals each monospecific for A. pleuropneumoniae serotype 2, 3, 5, 7, or 9 (in CFT). The calculation of notch boxes was performed according to the method of McGill et al. (11). The dashed line represents the cutoff set to 25 EU.
FIG. 2
FIG. 2
Distribution of ELISA activities of 22 A. pleuropneumoniae-infected herds (400 animals) (cross-hatched bars) and 13 A. pleuropneumoniae-free herds (216 animals) (striped bars).
See this image and copyright information in PMC

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