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. 1999 Aug;73(8):7061-4.
doi: 10.1128/JVI.73.8.7061-7064.1999.

The integration machinery of ZAM, a retroelement from Drosophila melanogaster, acts as a sequence-specific endonuclease

P Leblanc  1 , B Dastugue, C Vaury
Affiliations

The integration machinery of ZAM, a retroelement from Drosophila melanogaster, acts as a sequence-specific endonuclease

P Leblanc et al. J Virol. 1999 Aug.

Abstract

Retroviruses and retrotransposons insert into the host genome with no obvious sequence specificity. We examined the target sites of the retroelement ZAM by sequencing each host-ZAM junction in the genome of Drosophila melanogaster. Our overall data provide compelling evidence that ZAM integration machinery recognizes and leads to ZAM insertion into the sequence 5'-GCGCGCg-3'. This unique property of ZAM will facilitate the development of new tools to study the integration process of retroelements.

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Figures

FIG. 1
FIG. 1
(Upper panel) ZAM target duplication sequences contain an HhaI restriction site. Genomic DNAs of high- and low-copy-number strains were treated with the HhaI restriction endonuclease and hybridized to the LTR (A) or E6 (B) probe. (Lower panel) ZAM HhaI restriction map. Probes used for Southern analysis (LTR and E6 probes) and expected digested fragments are depicted below the restriction map. The HhaI restriction sites in shaded boxes are located in ZAM target duplication sequences.
FIG. 2
FIG. 2
(Upper panel) ZAM target duplication sequences contain a ThaI restriction site. Genomic DNAs of high- and low-copy-number strains were treated with the ThaI restriction endonuclease and hybridized to the LTR (A) or E6 (B) probe. (Lower panel) ZAM ThaI restriction map. Probes used for Southern analysis (LTR and E6 probes) and expected digested fragments are depicted below the restriction map. The ThaI restriction sites in shaded boxes are located in ZAM target duplication sequences.
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