Hymenolepis diminuta: praziquantel removal of adult tapeworms is followed by apoptotic down-regulation of mucosal mastocytosis
- PMID: 10403758
- DOI: 10.1006/expr.1999.4409
Hymenolepis diminuta: praziquantel removal of adult tapeworms is followed by apoptotic down-regulation of mucosal mastocytosis
Abstract
The rat tapeworm, Hymenolepis diminuta, induces mastocytosis, hypertrophy of enteric smooth muscle, alteration of enteric myoelectric activity, and slowed enteric transit of the rat host's intestine. This report examines the resolution of both tapeworm-induced mastocytosis and tissue changes during the period following removal of the tapeworm with Praziquantel (PZQ). The dynamics of the mucosal mast cell (MMC) population following removal of the tapeworms was assessed by histochemical identification of MMC and morphometric techniques. As a possible mechanism of MMC population regulation, MMC apoptosis was examined over the same experimental period using the in situ nick end labeling of fragmented DNA (TUNEL). Shifts in MMC numbers were correlated with functional and morphological changes of the intestine following removal of the adult-stage tapeworm. Ileal tissues from rats infected 32 days with H. diminuta (the beginning of plateau phase of tapeworm-induced chronic mastocytosis) were harvested 1, 2, 3, and 4 weeks after the PZQ treatment. Control ilea were obtained either from rats which were never infected and never treated with PZQ or from rats infected with H. diminuta for 32 days but not treated with PZQ. In order to detect MMC and apoptosis, tissue sections of ileum were doubled stained sequentially with Astra blue for MMC granules followed by a modification of the TUNEL technique. No alteration in MMC numbers were observed in PZQ-treated animals until 3 weeks after the removal of the tapeworms. The decline of MMC occurred in the mucosa and submucosa. MMC numbers first approached uninfected control levels at 4 weeks posttreatment. Coincident with the decline in mucosal MMC numbers, the rate of MMC entering apoptosis also declined. Simultaneously, ileal smooth muscle layers, hypertrophied by infection, and mucosal structures began the process of involution and atrophy. Apoptosis of MMC in the submucosa and muscularis mucosa was not detected. In conclusion, H. diminuta-elicited mastocytosis and increased thickness of both mucosa and muscularis externa do not begin a decline toward control values until 3 weeks after the parasites are gone and normal intestinal motility is restored. These data are consistent with the lack of MMC mediation of altered motility, and the decline in the rate of MMC apoptosis at 3 weeks post-PZQ suggests that apoptosis may play an important role in the involution of tapeworm-induced mastocytosis.
Copyright 1999 Academic Press.
Similar articles
-
Praziquantel treatment normalizes intestinal myoelectric alterations associated with Hymenolepis diminuta-infected rats.J Parasitol. 1995 Dec;81(6):979-84. J Parasitol. 1995. PMID: 8544075
-
Hymenolepsis diminuta: mucosal mastocytosis and intestinal smooth muscle hypertrophy occur in tapeworm-infected rats.Exp Parasitol. 1998 May;89(1):92-102. doi: 10.1006/expr.1998.4271. Exp Parasitol. 1998. PMID: 9603494
-
Ileal mucosal mast cell, eosinophil, and goblet cell populations during Hymenolepis diminuta infection of the rat.J Parasitol. 2001 Oct;87(5):1222-5. doi: 10.1645/0022-3395(2001)087[1222:IMMCEA]2.0.CO;2. J Parasitol. 2001. PMID: 11695409
-
Human infections by Hymenolepis diminuta in Europe: a case report and literature review.Trans R Soc Trop Med Hyg. 2024 Sep 2;118(9):580-588. doi: 10.1093/trstmh/trae037. Trans R Soc Trop Med Hyg. 2024. PMID: 38864212 Review.
-
Worldwide overview of human infections with Hymenolepis diminuta.Parasitol Res. 2020 Jul;119(7):1997-2004. doi: 10.1007/s00436-020-06663-x. Epub 2020 Mar 24. Parasitol Res. 2020. PMID: 32211990 Review.
Cited by
-
Antibody response in mice infected with Mesocestoides vogae (syn. Mesocestoides corti) tetrathyridia after treatment with praziquantel and liposomised glucan.Parasitol Res. 2007 May;100(6):1351-9. doi: 10.1007/s00436-006-0434-2. Epub 2007 Jan 25. Parasitol Res. 2007. PMID: 17252273
-
Increased glial-derived neurotrophic factor in the small intestine of rats infected with the tapeworm, Hymenolepis diminuta.Int J Exp Pathol. 2008 Dec;89(6):458-65. doi: 10.1111/j.1365-2613.2008.00606.x. Int J Exp Pathol. 2008. PMID: 19134055 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Research Materials