Identification of human rotavirus strains with the P[14] genotype by PCR
- PMID: 10405427
- PMCID: PMC85321
- DOI: 10.1128/JCM.37.8.2706-2708.1999
Identification of human rotavirus strains with the P[14] genotype by PCR
Abstract
A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
Figures


References
-
- Arista S, Giovannelli L, Passarani N, Titone L, Gerna G. Electropherotyping of human rotaviruses: an epidemiological survey of rotavirus infections in Sicily. Eur J Epidemiol. 1986;2:104–107. - PubMed
-
- Arista S, Giovannelli L, Pistoia D, Cascio A, Parea M, Gerna G. Electropherotypes, subgroups and serotypes of human rotavirus strains causing gastroenteritis in infants and young children in Palermo, Italy, from 1985 to 1989. Res Virol. 1990;141:435–446. - PubMed
-
- Ciarlet M, Estes M K, Conner M E. Comparative amino acid sequence analysis of the outer capsid protein VP4 from four lapine rotavirus strains reveals identity with genotype P[14] human rotaviruses. Arch Virol. 1997;142:1059–1069. - PubMed
-
- Gentsch J R, Woods P A, Ramachandran M, Das B K, Leite J P, Alfieri A, Kumar R, Bhan M K, Glass R I. Review of G and P typing results from a global collection of rotavirus strains: implications for vaccine development. J Infect Dis. 1996;174(Suppl. 1):S30–S36. - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources