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Comparative Study
. 1999 Aug;37(8):2709-14.
doi: 10.1128/JCM.37.8.2709-2714.1999.

Novel immunoblot assay using four recombinant antigens for diagnosis of Epstein-Barr virus primary infection and reactivation

M Buisson  1 , B Fleurent, M Mak, P Morand, L Chan, A Ng, M Guan, D Chin, J M Seigneurin
Affiliations
Comparative Study

Novel immunoblot assay using four recombinant antigens for diagnosis of Epstein-Barr virus primary infection and reactivation

M Buisson et al. J Clin Microbiol. 1999 Aug.

Abstract

A new immunoblot assay, composed of four Epstein-Barr virus (EBV)-encoded recombinant proteins (virus capsid antigen [VCA] p23, early antigen [EA] p138, EA p54, and EBNA-1 p72), was compared with an immunofluorescence assay on a total of 291 sera. The test was accurate in 94.5% of cases of primary EBV infection, while an immunoglobulin G anti-VCA p23 band with strong intensity correlated with reactivation.

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Figures

FIG. 1
FIG. 1
EBV IF interpretation algorithm established from routine laboratory experience. ∗, if the VCA IgG titer is ≥1,280, there is possible reactivation or chronic infection; ∗∗, if the EA IgG titer is ≥80, there is possible reactivation.
FIG. 2
FIG. 2
EBV blot interpretation algorithm established by this study. ∗, if the VCA p23 IgG titer is ≥3+, there is possible reactivation without EBNA. If there is a band intensity of 3+ or less, refer to IgM control band intensity of IgM assay.
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