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. 1999 Aug;10(8):2475-9.
doi: 10.1091/mbc.10.8.2475.

Compartmentalization of the erythrocyte membrane by the membrane skeleton: intercompartmental hop diffusion of band 3

M Tomishige  1 , A Kusumi
Affiliations
Free PMC article

Compartmentalization of the erythrocyte membrane by the membrane skeleton: intercompartmental hop diffusion of band 3

M Tomishige et al. Mol Biol Cell. 1999 Aug.
Free PMC article
No abstract available

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Figures

Figure 1
Figure 1
(A) The model to explain the confined diffusion of band 3 in the membrane (the membrane skeleton fence model). (B) Movement of gold particles attached to band 3 in the erythrocyte membrane, observed with a temporal resolution of 8 ms (4 times greater than the normal video rate).
Figure 2
Figure 2
Long-term observations of the movement of band 3 in the erythrocyte membrane. Images recorded every 250 ms using time-lapse video microscopy.
Figure 3
Figure 3
Movement of trypsin-cleaved band 3 was recorded with temporal resolutions of 8 and 0.89 ms.
Figure 4
Figure 4
Deformation of the membrane skeleton using optical tweezers. A 1-μm latex bead, coated with anti-band 3 IgG, bound multiple band 3 molecules, of which ∼30% are attached to the membrane skeleton. By dragging the latex bead, it was possible to deform the membrane skeleton, which can be observed by single particle tracking using gold particles specifically attached to spectrin on the cytoplasmic surface of the erythrocyte ghost membrane.
Figure 5
Figure 5
(A) Effect of lateral dragging of a 1-μm latex bead attached to the membrane skeleton on the movement of several band 3 molecules capable of undergoing hop diffusion. (B) The gold particles bound to band 3 undergoing hop diffusion followed the bead when it was dragged toward the left at a rate of 0.15 μm/s.
Figure 6
Figure 6
Effect of lateral dragging of a 1-μm latex bead attached to the membrane skeleton on the movement of a lipid that is located in the outer leaflet of the cell membrane.
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