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. 1999 Aug 17;96(17):9745-50.
doi: 10.1073/pnas.96.17.9745.

Radiation hybrid mapping of the zebrafish genome

N A Hukriede  1 , L Joly, M Tsang, J Miles, P Tellis, J A Epstein, W B Barbazuk, F N Li, B Paw, J H Postlethwait, T J Hudson, L I Zon, J D McPherson, M Chevrette, I B Dawid, S L Johnson, M Ekker
Affiliations

Radiation hybrid mapping of the zebrafish genome

N A Hukriede et al. Proc Natl Acad Sci U S A. .

Abstract

The zebrafish is an excellent genetic system for the study of vertebrate development and disease. In an effort to provide a rapid and robust tool for zebrafish gene mapping, a panel of radiation hybrids (RH) was produced by fusion of irradiated zebrafish AB9 cells with mouse B78 cells. The overall retention of zebrafish sequences in the 93 RH cell lines that constitute the LN54 panel is 22%. Characterization of the LN54 panel with 849 simple sequence length polymorphism markers, 84 cloned genes and 122 expressed sequence tags allowed the production of an RH map whose total size was 11,501 centiRays. From this value, we estimated the average breakpoint frequency of the LN54 RH panel to correspond to 1 centiRay = 148 kilobase. Placement of a group of 235 unbiased markers on the RH map suggests that the map generated for the LN54 panel, at present, covers 88% of the zebrafish genome. Comparison of marker positions in RH and meiotic maps indicated a 96% concordance. Mapping expressed sequence tags and cloned genes by using the LN54 panel should prove to be a valuable method for the identification of candidate genes for specific mutations in zebrafish.

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Figures

Figure 1
Figure 1
Comparison of RH and meiotic maps. For each linkage group (LG), the meiotic map is on the left and the RH map is on the right. Solid lines connect the same marker placed on both maps; dashed lines connect markers that fall into the same high-confidence bin. Markers depicted in black are SSLP markers, markers in red are cloned genes, and markers in green are ESTs. Below the map of each LG is the total distance estimate, in centiMorgans (cM) for the meiotic maps and cR for the RH maps. Four gaps remain on the RH maps, regions not linked at lods of five or higher; these gaps are depicted in red (LG 1, 20, 23, and 25). All placement markers are italicized. (Figure continues on opposite page.)
Figure 1
Figure 1
Comparison of RH and meiotic maps. For each linkage group (LG), the meiotic map is on the left and the RH map is on the right. Solid lines connect the same marker placed on both maps; dashed lines connect markers that fall into the same high-confidence bin. Markers depicted in black are SSLP markers, markers in red are cloned genes, and markers in green are ESTs. Below the map of each LG is the total distance estimate, in centiMorgans (cM) for the meiotic maps and cR for the RH maps. Four gaps remain on the RH maps, regions not linked at lods of five or higher; these gaps are depicted in red (LG 1, 20, 23, and 25). All placement markers are italicized. (Figure continues on opposite page.)
Figure 2
Figure 2
Average retention of the LN54 Panel.
See this image and copyright information in PMC

Comment in

  • Zebrafish genetics: the enigma of arrival.
    Fishman MC. Fishman MC. Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10554-6. doi: 10.1073/pnas.96.19.10554. Proc Natl Acad Sci U S A. 1999. PMID: 10485858 Free PMC article. Review. No abstract available.

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