[HIV-1 env glycoprotein gp120 and gp160 expressed in vaccinia virus system and their antigenicity analysis]

Abstract

Human immunodeficiency virus type 1 (HIV-1) is the principal causative agent of AIDS that is an important concerns to human being health. The most important antigen of HIV-1 is its envelope glycoprotein gp160 (gp120 + gp41). By means of genetic engineering techniques, HIV-1 gp120 and gp160 were constructed and expressed in the recombinant vaccinia virus system under the control of bacteriophage T7 promoter or vaccinia virus p7.5 promoter. The results showed that the recombinant gp120 and gp160 expressed in this system exist in the form as glycoproteins, resuming the native epitope conformation of the viruses from which they were derived. These recombinant gp120 and gp160 could be recognized by the virus-specific neutralizing monoclonal antibodies. The findings suggested that the recombinant HIV-1 gp120 and gp160 could be used as a candidate recombinant protein vaccines.