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. 1999 Sep;65(9):4245-57.
doi: 10.1128/AEM.65.9.4245-4257.1999.

Temperature-dependent fermentation of D-sorbitol in Escherichia coli O157:H7

O M Bouvet  1 , S Pernoud, P A Grimont
Affiliations

Temperature-dependent fermentation of D-sorbitol in Escherichia coli O157:H7

O M Bouvet et al. Appl Environ Microbiol. 1999 Sep.

Abstract

The influence of growth temperature on the ability to ferment D-sorbitol was investigated in Escherichia coli O157:H7. It was found that O157:H7 strains have a temperature-sensitive sorbitol phenotype. D-Sorbitol transport and sorbitol-6-phosphate dehydrogenase activities were expressed in sorbitol-fermenting cells grown at 30 degrees C but only at a low level at 40 degrees C. Sorbitol-positive variants able to transport D-sorbitol were easily selected at 30 degrees C from culture of Sor(-) E. coli O157:H7 strains.

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Figures

FIG. 1
FIG. 1
Anaerobic growth of Sor− and Sor+ strains of E. coli O157:H7 on d-sorbitol at 30 and 40°C. The means of at least three determinations are presented, and the standard deviation was 0.01 to 0.05. (A) Cells of Ec40 (circles) and its sorbitol Sor+ variant (triangles) were grown anaerobically at 30°C (open symbols) and at 40°C (filled symbols) on peptone broth medium containing 10 mM d-sorbitol. (B) Cells of 96336 (circles) and 75-88 strains (triangles) growing anaerobically at 30°C (open symbols) and at 40°C (filled symbols) on the same medium.
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