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Comparative Study
. 1999 Jul;84(4):803-6.

Speeds of actin translocation in vitro by myosins extracted from single rat muscle fibres of different types

M Canepari  1 , R Rossi, M A Pellegrino, C Reggiani, R Bottinelli
Affiliations
  • PMID: 10481236
Comparative Study

Speeds of actin translocation in vitro by myosins extracted from single rat muscle fibres of different types

M Canepari et al. Exp Physiol. 1999 Jul.

Abstract

As skeletal muscle fibres mostly express a single myosin isoform, they are a potential source of pure myosin isoforms. A technique is described that allows extraction and identification of pure myosin isoforms from single fibres, and testing of such myosins in an in vitro motility assay (IVMA). The results show that the extraction procedure does not alter myosin function and support the view that single fibres are reliable sources of purified myosin isoforms for IVMA.

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