doi: 10.1007/BF02816214.
A simple method for the isolation and purification of L-asparaginase from Enterobacter aerogenes
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- PMID: 10489691
- DOI: 10.1007/BF02816214
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A simple method for the isolation and purification of L-asparaginase from Enterobacter aerogenes
Folia Microbiol (Praha).
1999.
Abstract
L-Asparaginase from Enterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5-6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54%. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification of L-asparaginase. The choice of the column material was critical in the purification process.