[Hot start of the polymerase chain reaction using DNA helicase]
[Article in
Russian]
- PMID: 10495897
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[Hot start of the polymerase chain reaction using DNA helicase]
[Article in
Russian]
Bioorg Khim.
1999 May.
Abstract
A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture.
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