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. 1999 Oct;24(10):379-84.
doi: 10.1016/s0968-0004(99)01453-x.

The study of protein mechanics with the atomic force microscope

T E Fisher  1 , A F Oberhauser, M Carrion-Vazquez, P E Marszalek, J M Fernandez
Affiliations

The study of protein mechanics with the atomic force microscope

T E Fisher et al. Trends Biochem Sci. 1999 Oct.

Erratum in

  • Trends Biochem Sci 2000 Jan;25(1):6

Abstract

The unfolding and folding of single protein molecules can be studied with an atomic force microscope (AFM). Many proteins with mechanical functions contain multiple, individually folded domains with similar structures. Protein engineering techniques have enabled the construction and expression of recombinant proteins that contain multiple copies of identical domains. Thus, the AFM in combination with protein engineering has enabled the kinetic analysis of the force-induced unfolding and refolding of individual domains as well as the study of the determinants of mechanical stability.

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