Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1999 Jun;11(3):225-8.
doi: 10.1007/BF02788681.

Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction

X O Yang  1 , X D Huang, H L Zhao, J W Zhang
Affiliations

Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction

X O Yang et al. Mol Biotechnol. 1999 Jun.

Abstract

An efficient and simple method for removing known, abundant cDNA species from the cDNA pool of highly differentiated cells is reported. The method involves preparation of sscDNA, followed by dscDNA-synthesis of known, abundant cDNA species led by specific primers and removal of the synthesized dscDNA with hydroxyapatite. By using this method, the globin cDNAs were reduced to less than 10(-5) of their original abundance. The results suggest that this method may facilitate the isolation of new genes from specific cells or tissues.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2825-9 - PubMed
    1. Biotechniques. 1995 Oct;19(4):580-2, 584 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6 - PubMed
    1. Blood. 1994 Mar 1;83(5):1412-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2720-4 - PubMed

Publication types

MeSH terms

LinkOut - more resources