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. 1999 Jun;11(3):225-8.
doi: 10.1007/BF02788681.

Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction

X O Yang  1 , X D Huang, H L Zhao, J W Zhang
Affiliations

Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction

X O Yang et al. Mol Biotechnol. 1999 Jun.

Abstract

An efficient and simple method for removing known, abundant cDNA species from the cDNA pool of highly differentiated cells is reported. The method involves preparation of sscDNA, followed by dscDNA-synthesis of known, abundant cDNA species led by specific primers and removal of the synthesized dscDNA with hydroxyapatite. By using this method, the globin cDNAs were reduced to less than 10(-5) of their original abundance. The results suggest that this method may facilitate the isolation of new genes from specific cells or tissues.

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