An interpretation of the apparent dual specificity of some murine myeloma immunoglobulins with inulinbinding activity

Abstract

Five murine myeloma immunoglobulins have been studied for their binding with fructose-containing homopolysaccharides. The results indicate that the anti-(2 leads to 1)-D-fructofuranans are capable of binding (2 leads to 6)-D-fructofuranans with reduced affinity. An anti-(2 leads to 6)-D-fructofuranan (U10) was incapable of binding a (2 leads to 1)-D-fructofuranan. By using molecular models of fructofuranans of both linkage types, and a hypothetical molecular model of the combining region of E109, an anti-(2 leads to 1)-D-fructofuranan, a rationalization of these date can be proposed.