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Review
. 1999 Oct;43(10):2339-44.
doi: 10.1128/AAC.43.10.2339.

Lack of cell wall peptidoglycan versus penicillin sensitivity: new insights into the chlamydial anomaly

J M Ghuysen  1 , C Goffin
Affiliations
Review

Lack of cell wall peptidoglycan versus penicillin sensitivity: new insights into the chlamydial anomaly

J M Ghuysen et al. Antimicrob Agents Chemother. 1999 Oct.
No abstract available

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Figures

FIG. 1
FIG. 1
Lipid II as the immediate biosynthetic precursor of wall peptidoglycan in E. coli and putative glycanless wall polypeptide in C. trachomatis. G, N-acetylglucosamine; M, N-acetylmuramic acid; Dpm, diaminopimelic acid; thick bar, transmembrane C55 lipid. Reaction 1, transglycosylase-catalyzed synthesis of a glycosidic bond. Reaction 2, N-acetylmuramoyl-l-alanine-catalyzed hydrolysis of a d-lactoyl–l-alanine bond. Reactions 3 and 4, acylserine transferase-catalyzed transpeptidations at the expense of the d-alanyl–d-alanine bond of pentapeptide units. The amino acceptors of the transfer reactions are the amino group at the D center of meso-diaminopimelic acid in reaction 3 and the amidase-released l-alanine amino group in reaction 4. The product of reactions 1 and 3 is the wall peptidoglycan. The product of reactions 2, 3, and 4 is the putative wall polypeptide.
FIG. 2
FIG. 2
Modular design of the multimodular PBPs of classes A and B. Indicated are the positions of the five motifs of the transglycosylase n-PB module of class A PBPs (□) and the three motifs of the cell cycle n-PB module of class B PBPs (○). The intermodule junction sites (▵) are common to the PBPs of classes A and B.
FIG. 3
FIG. 3
Occurrence of motifs characteristic of class B PBPs along amino acid sequences of the 647-amino-acid (ORF D270-encoded) PBP and the 1,080-amino-acid (ORFD682-encoded) PBP of C. trachomatis (Ctr). E. coli (Eco) PBP 3 and PBP 2 are the prototypes of PBPs of subclasses B3 and B2, respectively (17). The intermotif distances are in numbers of amino acid residues.
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