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. 1999 Oct;65(10):4425-30.
doi: 10.1128/AEM.65.10.4425-4430.1999.

Identification of Enterococcus spp. with a biochemical key

A Manero  1 , A R Blanch
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Identification of Enterococcus spp. with a biochemical key

A Manero et al. Appl Environ Microbiol. 1999 Oct.

Abstract

A six-step biochemical key is presented for the identification of all recognized Enterococcus spp. The key consists of 12 tests, but no more than 6 are needed for the most complicated identification. The reliability of the key has been evaluated with collection type strains and clinical and environmental isolates. This key has fewer tests than those reported in previous studies. There is no commercial kit that includes the whole set of tests. However, some of the tests are included in enzyme activity-based kits that could be used with the proposed key. The key is designed for use in routine applications, especially in environmental and clinical studies with a high number of isolates.

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Figures

FIG. 1
FIG. 1
Identification key for Enterococcus spp. All species have a threshold of 100% except for E. hirae, E. durans, and E. avium, which present a threshold of 91.5, 97, and 87%, respectively. PYRase, pyrrolidonyl aminopeptidase.
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