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. 1999;77(2):88-96.
doi: 10.1159/000031082.

Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components

I MacGregor  1 , J Hope, G Barnard, L Kirby, O Drummond, D Pepper, V Hornsey, R Barclay, H Bessos, M Turner, C Prowse
Affiliations

Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components

I MacGregor et al. Vox Sang. 1999.

Abstract

Background and objectives: To quantify the cellular isoform of prion protein (PrP(c)) in human blood using a new time-resolved dissociation-enhanced fluoroimmunoassay (DELFIA).

Materials and methods: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrP(c) were analysed.

Results: 26. 5% of blood PrP(c) was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations).

Conclusion: The majority of blood PrP(c) is found in the platelet and plasma compartments.

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