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. 1999 Sep 10;458(1):27-31.
doi: 10.1016/s0014-5793(99)01119-9.

A 6 bp Z-DNA hairpin binds two Z alpha domains from the human RNA editing enzyme ADAR1

M Schade  1 , J Behlke, K Lowenhaupt, A Herbert, A Rich, H Oschkinat
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Free article

A 6 bp Z-DNA hairpin binds two Z alpha domains from the human RNA editing enzyme ADAR1

M Schade et al. FEBS Lett. .
Free article

Abstract

The Z alpha domain of the human RNA editing enzyme double-stranded RNA deaminase I (ADAR1) binds to left-handed Z-DNA with high affinity. We found by analytical ultracentrifugation and CD spectroscopy that two Z alpha domains bind to one d(CG)3T4(CG)3 hairpin which contains a stem of six base pairs in the Z-DNA conformation. Both wild-type Z alpha and a C125S mutant show a mean dissociation constant of 30 nM as measured by surface plasmon resonance and analytical ultracentrifugation. Our data suggest that short (> or = 6 bp) segments of Z-DNA within a gene are able to recruit two ADAR1 enzymes to that particular site.

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