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. 1999 Oct;9(10):989-93.
doi: 10.1101/gr.9.10.989.

A resource of mapped human bacterial artificial chromosome clones

V G Cheung  1 , H L Dalrymple, S Narasimhan, J Watts, G Schuler, A K Raap, M Morley, A Bruzel
Affiliations

A resource of mapped human bacterial artificial chromosome clones

V G Cheung et al. Genome Res. 1999 Oct.

Abstract

To date, despite the increasing number of genomic tools, there is no repository of ordered human BAC clones that covers entire chromosomes. This project presents a resource of mapped large DNA fragments that span eight human chromosomes at approximately 1-Mb resolution. These DNA fragments are bacterial artificial chromosome (BAC) clones anchored to sequence tagged site (STS) markers. This clone collection, which currently contains 759 mapped clones, is useful in a wide range of applications from microarray-based gene mapping to identification of chromosomal mutations. In addition to the clones themselves, we describe a database, GenMapDB (http://genomics.med.upenn.edu/genmapdb), that contains information about each clone in our collection.

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Figures

Figure 1
Figure 1
Schematic of the procedure for STS-based BAC mapping. (Step 1) Radiolabeled STS-probes are multiplexed and hybridized onto RPCI-11 segment 2 human male BAC library high-density filters. Liquid cultures of single colonies from positive clones are used to make dot blots. (Step 2) Individual probes are then hybridized onto the dot blots for verification and matching of clones to STS probes. (Steps 3 and 4) The mapped clones are then verified by two separate rounds of STS-content PCR, one round with liquid cultures of BAC clones (step 3) and another round with glycerol stocks of the mapped BAC clones (step 4).
Figure 2
Figure 2
FISH hybridization showing three BAC clones that mapped to Chromosome X. The three clones are 561-7J that mapped to DXS7274 (124 Mb from Xpter, Xq26 signal), 357-12N that mapped to DXS7503 (132 Mb from Xpter, Xq27 signal), and 490-11M that mapped to DXS7378 (151 Mb from Xpter, Xq28 signal).
Figure 3
Figure 3
Physical mapping information for chromosome 20. Shown is a part of a table in our database GenMapDB (http://genomics.med.upenn.edu/genmapdb), which includes STS markers, their physical addresses, and BAC clones corresponding to each STS-marker.
See this image and copyright information in PMC

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