Comparison of MB/BacT and BACTEC 460 TB systems for recovery of mycobacteria in a routine diagnostic laboratory
- PMID: 10523581
- PMCID: PMC85734
- DOI: 10.1128/JCM.37.11.3711-3712.1999
Comparison of MB/BacT and BACTEC 460 TB systems for recovery of mycobacteria in a routine diagnostic laboratory
Abstract
MB/BacT, BACTEC 460 TB, and Löwenstein-Jensen (LJ) medium were evaluated in parallel for recovery of mycobacteria from 3,700 continuous clinical specimens in a routine laboratory. Mycobacteria were identified from 123 (3.3%) specimens. The recovery rates for all mycobacteria by the different systems were 91.0, 73.0, and 53.6% for BACTEC 460 TB, MB/BacT, and LJ medium, respectively. The recovery rates for Mycobacterium tuberculosis complex were 97.1, 80. 2, and 67.6%, respectively. The lack of sensitivity of the MB/BacT system was more pronounced with smear-negative specimens and resulted in a failure to detect three patients with infectious tuberculosis.
Comment in
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The MB/BacT is a sensitive method of isolating Mycobacterium tuberculosis from clinical specimens in a laboratory with a low rate of isolation.J Clin Microbiol. 2000 Aug;38(8):3133-4. doi: 10.1128/JCM.38.8.3133-3134.2000. J Clin Microbiol. 2000. PMID: 11001664 Free PMC article. No abstract available.
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