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. 1999 Nov;37(11):3746-8.
doi: 10.1128/JCM.37.11.3746-3748.1999.

Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori

J R Gibson  1 , N A Saunders, B Burke, R J Owen
Affiliations

Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori

J R Gibson et al. J Clin Microbiol. 1999 Nov.

Abstract

A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori. Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method was rapid and reproducible, and it reduced PCR product contamination risk.

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Figures

FIG. 1
FIG. 1
Melting peaks obtained with a LightCycler for a clarithromycin-sensitive strain (–––), a resistant strain with the A2143C base change (…), and a resistant strain with either the A2143G or the A2144G base change (——). Values on the y axis are the first negative derivative of the change in fluorescence (dF) divided by the change in temperature (dT).
See this image and copyright information in PMC

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