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. 1979 Jan 2;167(3):251-8.
doi: 10.1007/BF00267416.

Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation

S Contente, D Dubnau

Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation

S Contente et al. Mol Gen Genet. .

Abstract

Transformation of competent cells of Bacillus subtilis with antibiotic resistance plasmid DNA has shown that (a) competence for plasmid and chromosomal DNA develops with similar kinetics; (b) DNA linearized with a variety of restriction endonucleases does not transform; (c) CCC plasmid DNA is inactivated for transformation by a single nick; (d) T4 ligase restores transforming activity to both nicked and linearized DNA; (E) CCC relaxed DNA is fully active in transformation; (f) the DNA concentration-dependence of plasmid transformation is first order; and (g) plasmid transformation proceeds with a low efficiency, requiring the uptake of 10(3) to 10(4) DNA molecules per transformant. Based on this information, a model for the processing of chromosomal, plasmid and transfecting DNA is proposed.

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