Organization of the coeruleo-vestibular pathway in rats, rabbits, and monkeys

Abstract

Inputs from locus coeruleus (LC) appear to be important for altering sensorimotor responses in situations requiring increase vigilance or alertness. This study documents the organization of coeruleo-vestibular pathways in rats, rabbits and monkeys. A lateral descending noradrenergic bundle (LDB) projects from LC to the superior vestibular nucleus (SVN) and rostral lateral vestibular nucleus (LVN). A medial descending noradrenergic bundle (MDB) projects from LC to LVN, the medial vestibular nucleus (MVN), group y and rostral nucleus prepositus hypoglossi (rNPH). There is a characteristic, specific pattern of innervation of vestibular nuclear regions across the three species. A quantitative analysis revealed four distinct innervation density levels (minimal, low, intermediate and high) across the vestibular nuclei. The densest plexuses of noradrenergic fibers were observed in the SVN and LVN. Less dense innervation was observed in the MVN, and minimal innervation was observed in the inferior vestibular nucleus (IVN). In monkeys and rabbits, rostral MVN contained a higher innervation density than the rat MVN. In monkeys, the rNPH also contained a dense plexus of fibers. Selective destruction of terminal LC projections (distal axons and terminals) by the neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4) resulted in a dramatic reduction of immunoreactive fibers within the vestibular nuclear complex of rats, suggesting that the source of these immunoreactive fibers is LC. Retrograde tracer injections into the vestibular nuclei resulted in labeled cells in the ipsilateral, caudal LC and adjacent nucleus subcoeruleus. It is hypothesized that the regional differences in noradrenergic innervation are a substrate for differentially altering vestibulo-ocular and vestibulo-spinal responses during changes in alertness or vigilance.