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. 1975 Nov;2(11):2049-54.
doi: 10.1093/nar/2.11.2049.

A new method of DNA denaturation mapping

D Johnson

A new method of DNA denaturation mapping

D Johnson. Nucleic Acids Res. 1975 Nov.

Abstract

Described is a new method for DNA denaturation mapping utilizing glyoxal (ethanedial) to stabilize the denatured regions. The extent of glyoxal reaction can be easily and sensitively measured using an assay based on the intercalation of ethidium into duplex DNA. Thus denturation maps can be produced in a controlled way under a wide variety of conditions.

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References

    1. Cold Spring Harb Symp Quant Biol. 1974;38:943-50 - PubMed
    1. Biochem Biophys Res Commun. 1974 Nov 27;61(2):396-403 - PubMed
    1. Can J Biochem. 1972 Feb;50(2):210-6 - PubMed
    1. Nucleic Acids Res. 1975 Apr;2(4):509-20 - PubMed
    1. Biochim Biophys Acta. 1971 Dec 30;254(3):380-8 - PubMed