A modified procedure for the preparation of di- and triribonucleotides from pancreatic ribonuclease digest of RNA
- PMID: 1052545
- PMCID: PMC343602
- DOI: 10.1093/nar/2.12.2355
A modified procedure for the preparation of di- and triribonucleotides from pancreatic ribonuclease digest of RNA
Abstract
A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH4HCO3.
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