Cerebral oxygenation during cardiopulmonary bypass measured by near-infrared spectroscopy: effects of hemodilution, temperature, and flow

Abstract

Objective: To determine the effects of hemodilution, PaCO2, PaO2, arterial pressure, and temperature on cerebral oxygenation during mild hypothermic cardiopulmonary bypass (CPB).

Participants: Fourteen patients electively scheduled for cardiac surgery.

Interventions: Oxyhemoglobin (HbO2), deoxyhemoglobin (Hb), hemoglobin differential (Hb-diff = HbO2-Hb), and oxidized cytochrome aa3 (CtO2) were measured with near-infrared spectroscopy (NIRS) during CPB.

Results: With onset of CPB, a significant decrease in HbO2 (median, -4.55 micromol/L; 25th to 75th percentile, -5.5 to -3.1; p < 0.05), Hb-diff (median, -3.88 micromol/L; 25th to 75th percentile, -4.7 to -1.9; p < 0.05), and CtO2 (median, -0.05 micromol/L; 25th to 75th percentile, -0.15 to 0; p < 0.001) occurred. The simultaneous decrease in arterial hemoglobin concentration (from 11.7 to 8.5 g/100 mL, p < 0.005) correlated significantly with changes in HbO2 (r2 = 0.71; p < 0.001), Hb-diff (r2 = 0.59; p < 0.005), and CtO2 (r2 = 0.57; p < 0.005). After 24 minutes of CPB, the largest decline in HbO2 (-5.03 micromol/L) and Hb-diff (-5.68 micromol/L) was recorded, whereas CtO2 showed no changes during cooling. During CPB, Hb and Hb-diff significantly correlated with the duration of CPB, PaO2 and PaCO2.

Conclusions: In early stages of CPB, a diminished cerebral oxygen supply was found, which may be caused by acute hemodilution. Despite an increased extraction of oxygen as demonstrated by the decrease in Hb-diff, cerebral energy balance reflected by CtO2 was maintained within a safe range during cooling. Because NIRS measures regional cerebral oxygenation, it is useful as an adjunct to global measures in the early noninvasive detection of cerebral hypoxia.