XRCC3 promotes homology-directed repair of DNA damage in mammalian cells
- PMID: 10541549
- PMCID: PMC317094
- DOI: 10.1101/gad.13.20.2633
XRCC3 promotes homology-directed repair of DNA damage in mammalian cells
Abstract
Homology-directed repair of DNA damage has recently emerged as a major mechanism for the maintenance of genomic integrity in mammalian cells. The highly conserved strand transferase, Rad51, is expected to be critical for this process. XRCC3 possesses a limited sequence similarity to Rad51 and interacts with it. Using a novel fluorescence-based assay, we demonstrate here that error-free homology-directed repair of DNA double-strand breaks is decreased 25-fold in an XRCC3-deficient hamster cell line and can be restored to wild-type levels through XRCC3 expression. These results establish that XRCC3-mediated homologous recombination can reverse DNA damage that would otherwise be mutagenic or lethal.
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