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. 1999 Nov;43(11):2798-800.
doi: 10.1128/AAC.43.11.2798.

Overexpression of Erg11p by the regulatable GAL1 promoter confers fluconazole resistance in Saccharomyces cerevisiae

D P Kontoyiannis  1 , N Sagar, K D Hirschi
Affiliations

Overexpression of Erg11p by the regulatable GAL1 promoter confers fluconazole resistance in Saccharomyces cerevisiae

D P Kontoyiannis et al. Antimicrob Agents Chemother. 1999 Nov.

Abstract

The contribution of the dosage of target enzyme P-450 14alpha-demethylase (14alphaDM) to fluconazole resistance in both Candida albicans and Saccharomyces cerevisiae remains unclear. Here, we show that overexpression of Saccharomyces P-450 14alphaDM in S. cerevisiae, under the control of the regulatable promoter GAL1, results in azole resistance.

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Figures

FIG. 1
FIG. 1
GAL1 ERG11 cDNA confers a galactose-dependent resistance to fluconazole. The growth responses of the GAL1 ERG11 cDNA clone in the following media are shown: SC-uracil–glucose (A), SC-uracil–glucose–fluconazole (128 μg/ml) (B), and SC-uracil–galactose–fluconazole (128 μg/ml) (C). The schematic drawing of a plate (lower right) indicates the location of the plated strains (1 to 6). The strains are described in the text.
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