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. 1999 Nov;65(11):5154-7.
doi: 10.1128/AEM.65.11.5154-5157.1999.

Recovery in embryonated eggs of viable but nonculturable Campylobacter jejuni cells and maintenance of ability to adhere to HeLa cells after resuscitation

J M Cappelier  1 , J Minet, C Magras, R R Colwell, M Federighi
Affiliations

Recovery in embryonated eggs of viable but nonculturable Campylobacter jejuni cells and maintenance of ability to adhere to HeLa cells after resuscitation

J M Cappelier et al. Appl Environ Microbiol. 1999 Nov.

Abstract

The existence of a viable but nonculturable (VBNC) state has been described for Campylobacter jejuni as it had been for a number pathogenic bacteria. Three C. jejuni human isolates were suspended in surface water and subsequently entered the VBNC state. After starvation for 30 days, VBNC cells were inoculated in the yolk sacs of embryonated eggs. Culturable cells were detected in a large proportion of the embryonated eggs inoculated with VBNC C. jejuni cells. Recovered cells kept their adhesion properties.

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Figures

FIG. 1
FIG. 1
Growth of a C. jejuni 85 cell suspension in microcosm water over a 30-day incubation period. Culturable-cell counts were obtained by spread plate counting. Active-cell counts were obtained by the CTC and DAPI staining technique, with cells being counted with an epifluorescence microscope. ■, culturable-cell count; ⧫, active-cell count; ▴, total-cell count.
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