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. 1999 Oct 8;858(1):103-7.
doi: 10.1016/s0021-9673(99)00827-4.

Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution

F Yang  1 , T Zhang, G Tian, H Cao, Q Liu, Y Ito
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Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution

F Yang et al. J Chromatogr A. .

Abstract

Analytical and preparative high-speed counter-current chromatography was successfully used for the isolation and purification of hydroxyanthraquinones from Rheum officinale Baill (Dahuang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion and aloe-emodin were isolated each at over 98% purity.

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